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Human Nesfatin-1/Nucleobindin-2/NUCB2 ELISA Kit

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EK1138

  • 96T ¥2480
  • 货期: 现货
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产品名称
Human Nesfatin-1/Nucleobindin-2/NUCB2 ELISA Kit
规格/价格
96T|2480
指标别名
DNA binding protein NEFA; Gastric cancer antigen Zg4; NEFA; NUCB2; nucleobindin 2
免疫原
E.coli, V25-L106
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名称
NUCB2
基因名全称
nucleobindin 2
Uniprot ID
P80303  
文献引用格式
Human Nesfatin-1/Nucleobindin-2/NUCB2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1138)
背景资料
Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2(NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.
显色时间
20-25分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1138-CAP96-well plate precoated with anti- human Nesfatin-1 antibody1
EK1138-STLyophilized recombinant human Nesfatin-1 standard2ng/tube×2
EK1138-DABiotinylated anti- human Nesfatin-1 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)58278993622861045
Standard deviation2.5517.5155.63.4120.5974.19
CV(%)4.46.35.65.57.27.1
典型数据示例
Concentration (pg/ml)031.262.512525050010002000
O.D.0.0250.0840.1330.2240.3650.711.1942.105
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants and Serum
实验时长
3.5h

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