产品名称
Human Nesfatin-1/Nucleobindin-2/NUCB2 ELISA Kit
指标别名
DNA binding protein NEFA; Gastric cancer antigen Zg4; NEFA; NUCB2; nucleobindin 2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
文献引用格式
Human Nesfatin-1/Nucleobindin-2/NUCB2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1138)
背景资料
Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2(NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1138-CAP | 96-well plate precoated with anti- human Nesfatin-1 antibody | 1 |
| EK1138-ST | Lyophilized recombinant human Nesfatin-1 standard | 2ng/tube×2 |
| EK1138-DA | Biotinylated anti- human Nesfatin-1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 58 | 278 | 993 | 62 | 286 | 1045 |
| Standard deviation | 2.55 | 17.51 | 55.6 | 3.41 | 20.59 | 74.19 |
| CV(%) | 4.4 | 6.3 | 5.6 | 5.5 | 7.2 | 7.1 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.025 | 0.084 | 0.133 | 0.224 | 0.365 | 0.71 | 1.194 | 2.105 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum