产品名称
Mouse S100A9 ELISA Kit
指标别名
60B8AG; CAGB; Calgranulin B; Calprotectin L1H subunit; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP 14; MRP14; NIF; P14; Protein S100 A9; S100A9
免疫原
Expression system for standard: E.coli; Immunogen sequence: A2-K113
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse S100A9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1152)
背景资料
Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin-B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1152-CAP | 96-well plate precoated with anti- mouse S100A9 antibody | 1 |
EK1152-ST | Lyophilized recombinant mouse S100A9 standard | 2ng/tube×2 |
EK1152-DA | Biotinylated anti- mouse S100A9 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 104 | 235 | 1022 | 110 | 231 | 1104 |
Standard deviation | 4.47 | 12.92 | 50.07 | 6.16 | 16.17 | 64.03 |
CV(%) | 4.3% | 5.5% | 4.9% | 5.6% | 7% | 5.8% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.097 | 0.113 | 0.139 | 0.204 | 0.395 | 0.679 | 1.256 | 2.215 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)