产品名称
Human CYR61/CCN1 ELISA Kit
指标别名
CCN family member 1; CCN1; CYR61; cysteine-rich protein 61; GIG1; IBP 10; IGF binding protein 10; IGFBP 10; IGFBP10; Protein CYR61; Protein GIG1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
cysteine-rich, angiogenic inducer, 61
文献引用格式
Human CYR61/CCN1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1203)
背景资料
Cysteine-rich angiogenic inducer 61(CYR61) or CCN family member 1(CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1203-CAP | 96-well plate precoated with anti- human CYR61 antibody | 1 |
| EK1203-ST | Lyophilized recombinant human CYR61 standard | 10ng/tube×2 |
| EK1203-DA | Biotinylated anti- human CYR61 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 61 | 248 | 814 | 59 | 242 | 806 |
| Standard deviation | 2.86 | 16.12 | 55.35 | 3.3 | 17.66 | 64.48 |
| CV(%) | 4.7 | 6.5 | 6.8 | 5.6 | 7.3 | 8 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.009 | 0.086 | 0.156 | 0.281 | 0.526 | 0.96 | 1.707 | 2.916 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk
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