Human Cystatin B/CSTB ELISA Kit

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EK1205

96T ￥2480
货期：现货

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产品名称

Human Cystatin B/CSTB ELISA Kit

规格/价格

96T|2480

指标别名

CPI B; CST6; CSTB; Cystatin B; cystatin B (stefin B); EPM1; PME; Stefin B; STFB

免疫原

E.coli, M2-F98

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

62.5 pg/ml - 4000 pg/ml

基因名称

CSTB

基因名全称

cystatin B (stefin B)

Uniprot ID

P04080

文献引用格式

Human Cystatin B/CSTB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1205)

背景资料

Cystatin B(CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK1205-CAP	96-well plate precoated with anti- human Cystatin B antibody	1
EK1205-ST	Lyophilized recombinant human Cystatin B standard	10ng/tube×2
EK1205-DA	Biotinylated anti- human Cystatin B antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	108	522	1824	110	532	1878
Standard deviation	7.77	32.88	131.32	9.57	40.96	153.99
CV(%)	7.2	6.3	7.2	8.7	7.7	8.2

典型数据示例

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.029	0.125	0.213	0.338	0.622	1.054	1.863	2.552

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

检验物种

human

检测方法

酶联免疫双抗夹心法

样本类型

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk

一抗

二抗

ELISA试剂盒

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Human Cystatin B/CSTB ELISA Kit

EK1205

产品简介 其他介绍 引用文献 相关产品

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

检验物种

检测方法

样本类型

实验时长

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Human Cystatin B/CSTB ELISA Kit

EK1205

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

All ELISA(1) All Species Human(1) 清除

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