产品名称
Mouse SAP/PTX2/APCS ELISA Kit
指标别名
9.5S alpha 1 glycoprotein; amyloid P component; serum; APCS; PTX2; SAP; Serum amyloid P component
免疫原
Expression system for standard: NS0; Immunogen sequence: Q21-D224
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Serum amyloid P-component
文献引用格式
Mouse SAP/PTX2/APCS ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1208)
背景资料
Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1208-CAP | 96-well plate precoated with anti- mouse SAP antibody | 1 |
| EK1208-ST0 | Lyophilized recombinant mouse SAP standard | 100ng/tube×2 |
| EK1208-DA | Biotinylated anti- mouse SAP antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 3608 | 12778 | 47613 | 3726 | 13449 | 43062 |
| Standard deviation | 144.32 | 715.56 | 2809.16 | 175.12 | 981.77 | 2626.78 |
| CV(%) | 4% | 5.6% | 5.9% | 4.7% | 7.3% | 6.1% |
典型数据示例
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.01 | 0.056 | 0.115 | 0.21 | 0.407 | 0.814 | 1.333 | 1.863 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)