产品名称
Human Lumican/LUM ELISA Kit
指标别名
KSPG lumican; LDC; LUM; lumican; SLRR2D
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human Lumican/LUM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1244)
背景资料
Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene(SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1244-CAP | 96-well plate precoated with anti- human Lumican antibody | 1 |
| EK1244-ST | Lyophilized recombinant human Lumican standard | 10ng/tube×2 |
| EK1244-DA | Biotinylated anti- human Lumican antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 349 | 1777 | 5103 | 367 | 1619 | 4802 |
| Standard deviation | 23.03 | 103.06 | 372.51 | 24.58 | 119.8 | 403.36 |
| CV(%) | 6.6 | 5.8 | 7.3 | 6.7 | 7.4 | 8.4 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.062 | 0.128 | 0.206 | 0.345 | 0.6256 | 1.112 | 1.758 | 2.274 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)