产品名称
Human Thioredoxin/TXN ELISA Kit
指标别名
ADF; ATL derived factor; SASP; Thioredoxin; TRDX; TRX; TRX1; TXN
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human Thioredoxin/TXN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1254)
背景资料
Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1254-CAP | 96-well plate precoated with anti- human Thioredoxin antibody | 1 |
| EK1254-ST | Lyophilized recombinant human Thioredoxin standard | 10ng/tube×2 |
| EK1254-DA | Biotinylated anti- human Thioredoxin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 300 | 1212 | 4585 | 329 | 1301 | 4269 |
| Standard deviation | 18.6 | 83.62 | 183.4 | 20.72 | 89.76 | 204.91 |
| CV(%) | 6.2 | 6.9 | 4 | 6.3 | 6.9 | 4.8 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.019 | 0.098 | 0.157 | 0.275 | 0.476 | 0.857 | 1.532 | 2.324 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)