产品名称
Mouse CD70 ELISA Kit
指标别名
CD27 L; CD27 ligand; CD27L; CD27LG; CD70; CD70 antigen; CD70 molecule; TNFSF7
免疫原
Expression system for standard: NS0; Immunogen sequence: S45-P195
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse CD70 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1310)
背景资料
CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1310-CAP | 96-well plate precoated with anti- mouse CD70 antibody | 1 |
| EK1310-ST | Lyophilized recombinant mouse CD70 standard | 10ng/tube×2 |
| EK1310-DA | Biotinylated anti- mouse CD70 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 79 | 248 | 791 | 80 | 240 | 839 |
| Standard deviation | 3.71 | 18.35 | 60.9 | 3.92 | 19.92 | 75.51 |
| CV(%) | 4.7% | 7.4% | 7.7% | 4.9% | 8.3% | 9% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.063 | 0.142 | 0.206 | 0.315 | 0.514 | 0.785 | 1.327 | 2.188 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)