产品名称
Human IGF2R ELISA Kit
指标别名
CD222; CI Man 6 P receptor; CI MPR; CIMPR; IGF II receptor; IGF2R; IGF2R-Specific; Lysosome Marker; M6P R; M6P/IGF2 receptor; M6P/IGF2R; M6PR; MPR 300; MPR1; MPRI
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
insulin-like growth factor 2 receptor
文献引用格式
Human IGF2R ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1326)
背景资料
Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1326-CAP | 96-well plate precoated with anti- human IGF2R antibody | 1 |
| EK1326-ST | Lyophilized recombinant human IGF2R standard | 10ng/tube×2 |
| EK1326-DA | Biotinylated anti- human IGF2R antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 249 | 1351 | 4462 | 248 | 1314 | 4363 |
| Standard deviation | 9.96 | 77 | 196.32 | 13.64 | 95.92 | 253.05 |
| CV(%) | 4 | 5.7 | 4.4 | 5.5 | 7.3 | 5.8 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.061 | 0.13 | 0.193 | 0.292 | 0.586 | 0.966 | 1.594 | 2.053 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)