产品名称
Mouse CXCL3 ELISA Kit
指标别名
C-X-C motif chemokine ligand 3; CINC-2b; GRO3; GROg; MIP-2b; MIP2B; SCYB3; CXCL3
免疫原
Expression system for standard: E.coli; Immunogen sequence: S32-S100
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-X-C motif chemokine 3
文献引用格式
Mouse CXCL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1364)
背景资料
Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1364-CAP | 96-well plate precoated with anti- mouse CXCL3 antibody | 1 |
| EK1364-ST | Lyophilized recombinant mouse CXCL3 standard | 10ng/tube×2 |
| EK1364-DA | Biotinylated anti- mouse CXCL3 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 35 | 205 | 489 | 35 | 202 | 512 |
| Standard deviation | 1.61 | 8.81 | 21.02 | 1.71 | 12.12 | 22.01 |
| CV(%) | 4.6% | 4.3% | 4.3% | 4.9% | 6% | 4.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.011 | 0.072 | 0.13 | 0.255 | 0.505 | 0.9 | 1.493 | 2.261 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)