产品名称
Mouse Tissue factor/F3 ELISA Kit
指标别名
CD142; Coagulation factor III; F3; TF; TFA; Thromboplastin; Tissue factor
免疫原
Expression system for standard: NS0; Immunogen sequence: A29-E251
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Tissue factor/F3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1367)
背景资料
Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1367-CAP | 96-well plate precoated with anti- mouse Tissue factor antibody | 1 |
| EK1367-ST | Lyophilized recombinant mouse Tissue factor standard | 10ng/tube×2 |
| EK1367-DA | Biotinylated anti- mouse Tissue factor antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 31 | 167 | 444 | 32 | 169 | 432 |
| Standard deviation | 1.3 | 9.18 | 32.41 | 1.5 | 10.47 | 35.85 |
| CV(%) | 4.2% | 5.5% | 7.3% | 4.7% | 6.2% | 8.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.017 | 0.064 | 0.127 | 0.248 | 0.48 | 0.822 | 1.297 | 2.024 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Cell Lysates