指标别名
CD44; CD44 antigen; CDW44; CSPG8; ECMR III; Epican; HCELL; Heparan sulfate proteoglycan; Hermes antigen; HUTCH I; Hyaluronate receptor; IN; MC56; MDU2; MDU3; MIC4; MUTCH I; PGP 1; PGP I; Pgp1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I
免疫原
Expression system for standard: NS0; Immunogen sequence: Q22-E271
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
CD44 molecule(Indian blood group)
文献引用格式
Rat CD44 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1417)
背景资料
CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1417-CAP | 96-well plate precoated with anti- Rat CD44 antibody | 1 |
| EK1417-ST | Lyophilized recombinant rat CD44 standard | 20ng/tube×2 |
| EK1417-DA | Biotinylated anti- rat CD44 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 481 | 1665 | 7855 | 452 | 1537 | 7710 |
| Standard deviation | 28.37 | 129.87 | 439.88 | 32.54 | 136.79 | 516.57 |
| CV(%) | 5.9% | 7.8% | 5.6% | 7.2% | 8.9% | 6.7% |
典型数据示例
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.012 | 0.093 | 0.14 | 0.251 | 0.443 | 0.871 | 1.433 | 2.321 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
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