Human Caspase 8/CASP8 ELISA Kit

96T/2480

Apoptotic protease Mch 5; Casp 8; CASP8; CASP-8; Caspase 8; caspase8; FADD like ICE; FLICE; ICE like apoptotic protease 5; MCH5

E.coli,S217-D384&L385-D479

There is no detectable cross-reactivity with other relevant proteins.

31.2 pg/ml - 2000 pg/ml

CASP8

caspase 8

Q14790  

Human Caspase 8/CASP8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1426)

Caspase-8 is a caspase protein, encoded by the CASP8 gene. It is mapped to human chromosome 2q33-q34 and mouse chromosome 1B-proximal C. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. And this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

20-25分钟

Catalog number	Description	Quantity
EK1426-CAP	96-well plate precoated with anti- human Caspase 8 antibody	1
EK1426-ST	Lyophilized recombinant human Caspase 8 standard	10ng/tube×2
EK1426-DA	Biotinylated anti- human Caspase 8 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	89	257	911	90	251	854
Standard deviation	5.69	16.44	46.46	6.57	20.58	46.11
CV(%)	6.4	6.4	5.1	7.3	8.2	5.4

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.003	0.067	0.111	0.218	0.407	0.820	1.339	2.191

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatantsand Serum

3.5h