产品名称
Mouse PDL1/CD274 ELISA Kit
指标别名
B7 H; B7 H1; B7 homolog 1; B7H1; CD274; CD274 molecule; PD L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1/CD274; Programmed death ligand 1
免疫原
Expression system for standard: NS0; Immunogen sequence: F19-H239
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Programmed cell death 1 ligand 1
文献引用格式
Mouse PDL1/CD274 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1450)
背景资料
Programmed death-ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1450-CAP | 96-well plate precoated with anti- mouse PD-L1 antibody | 1 |
| EK1450-ST | Lyophilized recombinant mouse PD-L1 standard | 10ng/tube×2 |
| EK1450-DA | Biotinylated anti- mouse PD-L1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 76 | 283 | 883 | 72 | 304 | 877 |
| Standard deviation | 5.16 | 21.79 | 49.44 | 5.04 | 29.18 | 51.74 |
| CV(%) | 6.8% | 7.7% | 5.6% | 7% | 9.6% | 5.9% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.056 | 0.099 | 0.136 | 0.201 | 0.348 | 0.6254 | 1.135 | 1.946 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
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