产品名称
Human CA125/MUC16 ELISA Kit
指标别名
CA 125; CA125; MUC 16; MUC16; MUC16; CA125; Mucin 16
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
mucin 16, cell surface associated
文献引用格式
Human CA125/MUC16 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1471)
背景资料
CA-125, also known as mucin 16 or MUC16, is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family glycoproteins. It is a component of the ocular surface, the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1471-CAP | 96-well plate precoated with anti- human CA125 antibody | 1 |
| EK1471-ST | Lyophilized recombinant human CA125 standard | 1ng/tube×2 |
| EK1471-DA | Biotinylated anti- human CA125 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 34 | 255 | 392 | 32 | 259 | 370 |
| Standard deviation | 1.36 | 20.14 | 17.64 | 1.47 | 21.75 | 21.09 |
| CV(%) | 4 | 7.9 | 4.5 | 4.6 | 8.4 | 5.7 |
典型数据示例
| Concentration (pg/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.056 | 0.099 | 0.142 | 0.201 | 0.348 | 0.6254 | 1.135 | 1.946 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk