产品名称
Human SFRP5 ELISA Kit
指标别名
FRP1b; FRP 1b; FRP-1b; SARP3; SARP 3; SARP-3; SFRP5; SFRP 5; SFRP-5
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
secreted frizzled-related protein 5
文献引用格式
Human SFRP5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1472)
背景资料
Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1472-CAP | 96-well plate precoated with anti- human SFRP5 antibody | 1 |
| EK1472-ST | Lyophilized recombinant human SFRP5 standard | 10ng/tube×2 |
| EK1472-DA | Biotinylated anti- human SFRP5 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 329 | 1068 | 4969 | 309 | 1159 | 5218 |
| Standard deviation | 20.39 | 69.42 | 203.72 | 21.93 | 96.19 | 250.46 |
| CV(%) | 6.2 | 6.5 | 4.1 | 7.1 | 8.3 | 4.8 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.005 | 0.064 | 0.119 | 0.219 | 0.407 | 0.78 | 1.442 | 2.017 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)