Human Pan Trypsin ELISA Kit

96T|2480

serine protease 1; TRP1; TRY1; TRY4; TRYP1; serine protease 3; MTG; PRSS4; T9; TRY3; TRY4; PRSS1/2/3

NSO, A16-S247

There is no detectable cross-reactivity with other relevant proteins.

31.2 pg/ml - 2000 pg/ml

PRSS1/2/3

protease, serine 1/2/3

P07477/P07478/P35030  

Human Pan Trypsin ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1531)

Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Tryptic digestion is a necessary step in protein absorption as proteins are generally too large to be absorbed through the lining of the small intestine. Additionally, Trypsin is produced as the inactive zymogen trypsinogen in the pancreas. When the pancreas is stimulated by cholecystokinin, it is then secreted into the first part of the small intestine (the duodenum) via the pancreatic duct. Once in the small intestine, the enzyme enteropeptidase activates trypsinogen into trypsin by proteolytic cleavage. Auto catalysis can happen with trypsin using trypsinogen as the substrate. This activation mechanism is common for most serine proteases, and serves to prevent autodegradation of the pancreas.

15-20分钟

Catalog number	Description	Quantity
EK1531-CAP	96-well plate precoated with anti- human Trypsin antibody	1
EK1531-ST	Lyophilized recombinant human Trypsin standard	10ng/tube×2
EK1531-DA	Biotinylated anti- human Trypsin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	85	261	887	82	235	820
Standard deviation	6.03	20.09	66.52	6.88	22.32	64.78
CV(%)	7.1	7.7	7.5	8.4	9.5	7.9

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.047	0.117	0.178	0.287	0.429	0.724	1.255	2.087

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin)

3.5h