产品名称
Mouse GREM2 ELISA Kit
指标别名
CKTSF1B2; DAN domain family member 3; DAND3; GREM2; Gremlin 2; PRDC
免疫原
Expression system for standard: E.coli; Immunogen sequence: R22-Q168
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse GREM2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1532)
背景资料
GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1532-CAP | 96-well plate precoated with anti- mouse GREM2 antibody | 1 |
| EK1532-ST | Lyophilized recombinant mouse GREM2 standard | 10ng/tube×2 |
| EK1532-DA | Biotinylated anti- mouse GREM2 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 95 | 279 | 843 | 99 | 268 | 803 |
| Standard deviation | 5.6 | 13.67 | 34.56 | 6.03 | 18.22 | 35.33 |
| CV(%) | 5.9% | 4.9% | 4.1% | 6.1% | 6.8% | 4.4% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.02 | 0.093 | 0.156 | 0.273 | 0.493 | 0.895 | 1.656 | 2.725 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)