产品名称
Human Syndecan-3/SDC3 ELISA Kit
指标别名
KIAA0468; N syndecan; SDC3; SDCN; SYND3; syndecan 3; Syndecan3; Syndecan-3
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human Syndecan-3/SDC3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1555)
背景资料
Syndecan-3 is a protein that in humans is encoded by the SDC3 gene. The protein encoded by this gene belongs to the syndecan proteoglycan family, which was assigned to human chromosome 1p35.2, and it showed that in the mouse the Synd3 gene maps to chromosome 4 near the Lmyc gene. The transfection of SDC3 resulted in the formation of long filopodia-like structures, microspikes, and varicosities in several cell lines. Besides, the gene plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1555-CAP | 96-well plate precoated with anti- human Syndecan-3 antibody | 1 |
| EK1555-ST | Lyophilized recombinant human Syndecan-3 standard | 10ng/tube×2 |
| EK1555-DA | Biotinylated anti- human Syndecan-3 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 237 | 1952 | 5230 | 246 | 1947 | 5345 |
| Standard deviation | 13.27 | 130.78 | 287.65 | 17.22 | 157.7 | 379.49 |
| CV(%) | 5.6 | 6.7 | 5.5 | 7 | 8.1 | 7.1 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.053 | 0.101 | 0.149 | 0.229 | 0.405 | 0.749 | 1.406 | 2.096 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)