产品名称
Human S100A8 ELISA Kit
指标别名
60B8AG; CAGA; Calgranulin A; Calprotectin L1L subunit; CFAG; CGLA; CP 10; Cystic fibrosis antigen; L1Ag; MA387; MIF; MRP 8; MRP8; NIF; P8; Protein S100 A8; S100A8; Urinary stone protein band A
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
S100 calcium binding protein A8
文献引用格式
Human S100A8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1558)
背景资料
S100 calcium-binding protein A8 (S100A8), also known as calgranulin A, is a protein that in humans is encoded by the S100A8 gene. It is mapped to chromosome 1q21.3 based on an alignment of the S100A8 sequence with the genomic sequence. The proteins S100A8 and S100A9 form a heterodimer called calprotectin which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. This gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1558-CAP | 96-well plate precoated with anti- human S100A8 antibody | 1 |
EK1558-ST | Lyophilized recombinant human S100A8 standard | 2ng/tube×2 |
EK1558-DA | Biotinylated anti- human S100A8 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 78 | 221 | 906 | 80 | 240 | 836 |
Standard deviation | 4.75 | 9.28 | 36.24 | 5.68 | 10.08 | 43.47 |
CV(%) | 6.1 | 4.2 | 4 | 7.1 | 4.2 | 5.2 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.024 | 0.059 | 0.081 | 0.139 | 0.260 | 0.518 | 1.239 | 2.236 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)