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Human S100A8 ELISA Kit

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EK1558

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human S100A8 ELISA Kit
规格/价格
96T/2480
指标别名
60B8AG; CAGA; Calgranulin A; Calprotectin L1L subunit; CFAG; CGLA; CP 10; Cystic fibrosis antigen; L1Ag; MA387; MIF; MRP 8; MRP8; NIF; P8; Protein S100 A8; S100A8; Urinary stone protein band A
免疫原
E.coli,L2-E93
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名称
S100A8
基因名全称
S100 calcium binding protein A8
Uniprot ID
P05109  
文献引用格式
Human S100A8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1558)
背景资料
S100 calcium-binding protein A8 (S100A8), also known as calgranulin A, is a protein that in humans is encoded by the S100A8 gene. It is mapped to chromosome 1q21.3 based on an alignment of the S100A8 sequence with the genomic sequence. The proteins S100A8 and S100A9 form a heterodimer called calprotectin which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. This gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1558-CAP96-well plate precoated with anti- human S100A8 antibody1
EK1558-STLyophilized recombinant human S100A8 standard2ng/tube×2
EK1558-DABiotinylated anti- human S100A8 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)7822190680240836
Standard deviation4.759.2836.245.6810.0843.47
CV(%)6.14.247.14.25.2
典型数据示例
Concentration(pg/ml)031.262.512525050010002000
O.D.0.0240.0590.0810.1390.2600.5181.2392.236
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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