产品名称
Human HGFA/HGFAC ELISA Kit
指标别名
HGF activator; HGFA; HGFAC
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
文献引用格式
Human HGFA/HGFAC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1592)
背景资料
Hepatocyte growth factor activator, also known as HGFA, is a protein that in humans is encoded by the HGFAC gene. This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1592-CAP | 96-well plate precoated with anti- human HGFA antibody | 1 |
| EK1592-ST | Lyophilized recombinant human HGFA standard | 10ng/tube×2 |
| EK1592-DA | Biotinylated anti- human HGFA antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 62 | 304 | 830 | 60 | 296 | 836 |
| Standard deviation | 3.1 | 20.06 | 52.29 | 3.9 | 22.79 | 59.35 |
| CV(%) | 5 | 6.6 | 6.3 | 6.5 | 7.7 | 7.1 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.019 | 0.074 | 0.121 | 0.198 | 0.354 | 0.6252 | 1.02 | 1.813 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)