产品名称
Rat TrkC/NTRK3 ELISA Kit
指标别名
GP145 TrkC; gp145(trkC); NT 3 growth factor receptor; NTRK3; TrkC; TrkC tyrosine kinase
免疫原
Expression system for standard: NS0; Immunogen sequence: C32-T429
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
neurotrophic receptor tyrosine kinase 3
文献引用格式
Rat TrkC/NTRK3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1660)
背景资料
NTRK3 (Neurotrophic Tyrosine Kinase Receptor Type 3), also known as TRKC, is a protein that in humans is encoded by the NTRK3 gene. By PCR analysis of a somatic cell hybrid panel and by fluorescence in situ hybridization with the cDNA clone, it mapped the NTRK3 gene to 15q24-q25. TRKC, a member of the TRK family of tyrosine protein kinase genes. They found that TRKC is preferentially expressed in the brain; in situ hybridization studies showed transcripts in the hippocampus, cerebral cortex, and the granular cell layer of the cerebellum. By functional studies in HeLa cells, 5 miRNAs regulate the truncated form of NTRK3.
试剂盒内容
Catalog number | Description | Quantity |
EK1660-CAP | 96-well plate precoated with anti- rat TRKC antibody | 1 |
EK1660-ST | Lyophilized recombinant rat TRKC standard | 10ng/tube×2 |
EK1660-DA | Biotinylated anti- rat TRKC antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 290 | 1865 | 4243 | 262 | 1846 | 4355 |
Standard deviation | 11.6 | 134.28 | 182.44 | 13.88 | 138.45 | 226.46 |
CV(%) | 4% | 7.2% | 4.3% | 5.3% | 7.5% | 5.2% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.046 | 0.133 | 0.215 | 0.367 | 0.657 | 1.006 | 1.669 | 2.392 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Cell Lysates