Catalog number | Description | Quantity |
---|---|---|
EK1688-CAP | 96-well plate precoated with anti- human Klotho antibody | 1 |
EK1688-ST | Lyophilized recombinant human Klotho standard | 10ng/tubex2 |
EK1688-DA | Biotinylated anti- human Klotho antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 308 | 1224 | 5567 | 337 | 1220 | 5449 |
Standard deviation | 15.4 | 79.56 | 239.38 | 20.55 | 102.48 | 321.49 |
CV(%) | 5 | 6.5 | 4.3 | 6.1 | 8.4 | 5.9 |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.055 | 0.073 | 0.100 | 0.141 | 0.231 | 0.481 | 1.089 | 1.920 |
Human Klotho ELISA Kit standard curve
Human Klotho ELISA Kit standard curve