产品名称
Human AMICA/JAML ELISA Kit
指标别名
AMICA; AMICA1; CREA7 1; CREA7 4; Gm638; JAML
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
junction adhesion molecule like
文献引用格式
Human AMICA/JAML ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1730)
背景资料
JAML or Junctional Adhesion Molecule-Like, or AMICA1 is a JAM transmembrane protein family member. This gene is mapped to 11q23.3. It is composed of two extracellular immunoglobulin-like domains, a membrane-spanning region, and a cytoplasmic tail involved in activation signaling. A known ligand of JAML is Coxsackie virus and Adenovirus Receptor (CXADR in humans and CAR in mice) which has been shown to localize to the tight junctions of epithelial cells. JAML-mediated activation of CAR is required for neutrophil extravasation in addition to other leukocyte/epithelial cell interaction models. Other members of the JAM family of transmembrane proteins include JAM1, JAM2 and JAM3.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1730-CAP | 96-well plate precoated with anti- human AMICA/JAML antibody | 1 |
EK1730-ST | Lyophilized recombinant human AMICA/JAML standard | 10ng/tube×2 |
EK1730-DA | Biotinylated anti- human AMICA/JAML antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 106 | 451 | 1179 | 110 | 500 | 1230 |
Standard deviation | 4.45 | 20.8 | 62.5 | 7.04 | 34 | 88.56 |
CV(%) | 4.2 | 4.6 | 5.3 | 6.4 | 6.8 | 7.2 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.143 | 0.201 | 0.267 | 0.412 | 0.599 | 0.962 | 1.603 | 2.304 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate )