产品名称
Human JAM-B/JAM2 ELISA Kit
指标别名
C21orf43; CD322; JAM 2; JAM B; JAM2; JAMB; junctional adhesion molecule 2; Junctional adhesion molecule B; PRO245; VE JAM; VEJAM
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
junctional adhesion molecule 2
文献引用格式
Human JAM-B/JAM2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1842)
背景资料
Junctional adhesion molecule B is a protein that in humans is encoded by the JAM2 gene. It is mapped to 21q21.3. This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1842-CAP | 96-well plate precoated with anti- human JAM-B/JAM2 antibody | 1 |
EK1842-ST | Lyophilized recombinant human JAM-B/JAM2 standard | 10ng/tube×2 |
EK1842-DA | Biotinylated anti- human JAM-B/JAM2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 27 | 190 | 408 | 31 | 213 | 467 |
Standard deviation | 1.13 | 12.73 | 22.44 | 2.11 | 12.57 | 28.49 |
CV(%) | 4.2 | 6.7 | 5.5 | 6.8 | 5.9 | 6.1 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.012 | 0.147 | 0.264 | 0.463 | 0.819 | 1.613 | 2.165 | 2.793 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)