Catalog number | Description | Quantity |
---|---|---|
EK1982-CAP | 96-well plate precoated with anti- human CD300F antibody | 1 |
EK1982-ST | Lyophilized recombinant human CD300F standard | 10ng/tube×2 |
EK1982-DA | Biotinylated anti- human CD300F antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 60 | 272 | 844 | 67 | 385 | 987 |
Standard deviation | 3.72 | 14.42 | 39.67 | 4.09 | 28.49 | 50.34 |
CV(%) | 6.2 | 5.3 | 4.7 | 6.1 | 7.4 | 5.1 |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.024 | 0.117 | 0.203 | 0.382 | 0.766 | 1.049 | 1.787 | 2.421 |
Human CD300F ELISA Kit Standard Curve
Human CD300F ELISA Kit Standard Curve