产品名称
Rat ANGPTL7 ELISA Kit
指标别名
angiopoietin like 7; Angiopoietin like factor; Angiopoietin like protein 7; Angiopoietin related protein 7; ANGPTL7; AngX; CDT6; RP4 647M16.2
免疫原
Expression system for standard: CHO; Immunogen sequence: Q22-P337
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
93.7 pg/ml - 6000 pg/ml
基因名全称
angiopoietin-like 7
文献引用格式
Rat ANGPTL7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2037)
背景资料
Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 1p36.22. Angiopoietin-like Protein 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted, 4550 kDa glycoprotein member of the angiopoietinlike family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera. Its production is upregulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. ANGPTL7 expression is upregulated under conditions of increased ocular pressure.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1628-CAP | 96-well plate precoated with anti- Rat ANGPTL7 antibody | 1 |
| EK1628-ST | Lyophilized recombinant Rat ANGPTL7 standard | 10 ng/tube×2 |
| EK1628-DA | Biotinylated anti- Rat ANGPTL7 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 158 | 630 | 1953 | 106 | 742 | 2900 |
| Standard deviation | 6.87 | 24.07 | 71.87 | 6.44 | 58.10 | 150.51 |
| CV (%) | 4.4% | 3.8% | 3.7% | 6.1% | 7.8% | 5.2% |
典型数据示例
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.057 | 0.11 | 0.158 | 0.245 | 0.439 | 0.736 | 1.235 | 2.017 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA).