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Human HEXA ELISA Kit

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EK2215

  • 96T ¥2480
  • 货期: 现货

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产品简介>

产品名称
Human HEXA ELISA Kit
规格/价格
96T/2480
指标别名
HEXA; Hexosaminidase subunit A; TSD
免疫原
Expression system for standard: NS0; Immunogen sequence: M1-T529
检测范围
312 pg/ml - 20000 pg/ml
基因名称
HEXA
基因名全称
hexosaminidase subunit alpha
Uniprot ID
P06865  
文献引用格式
Human HEXA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2215)
背景资料
Hexosaminidase A (alpha polypeptide), also known as HEXA, is an enzyme that in humans is encoded by the HEXA gene, located on the 15th chromosome. This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene lead to an accumulation of GM2 ganglioside in neurons, the underlying cause of neurodegenerative disorders termed the GM2 gangliosidoses, including Tay-Sachs disease (GM2-gangliosidosis type I). Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK2215-CAP96-well plate precoated with anti- Human HEXA ELISA Kit A antibody1
EK2215-STLyophilized recombinant Human HEXA ELISA Kit A standard1ng/tube×2
EK2215 -DABiotinylated anti- Human HEXA ELISA Kit A antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)5281757495555216944751
Standard deviation20.5989.61237.8428.7076.23242.3
CV(%)3.95.14.85.24.55.1
典型数据示例
Concentration(pg/ml)03126251,2502,5005,00010,00020,000
O.D.0.0250.0680.1450.2970.4610.9551.4322.077
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
蛋白名全称
Beta-hexosaminidase subunit alpha
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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