产品名称
Human HEXA ELISA Kit
指标别名
HEXA; Hexosaminidase subunit A; TSD
免疫原
Expression system for standard: NS0; Immunogen sequence: M1-T529
检测范围
312 pg/ml - 20000 pg/ml
基因名全称
hexosaminidase subunit alpha
文献引用格式
Human HEXA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2215)
背景资料
Hexosaminidase A (alpha polypeptide), also known as HEXA, is an enzyme that in humans is encoded by the HEXA gene, located on the 15th chromosome. This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene lead to an accumulation of GM2 ganglioside in neurons, the underlying cause of neurodegenerative disorders termed the GM2 gangliosidoses, including Tay-Sachs disease (GM2-gangliosidosis type I). Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK2215-CAP | 96-well plate precoated with anti- Human HEXA ELISA Kit A antibody | 1 |
EK2215-ST | Lyophilized recombinant Human HEXA ELISA Kit A standard | 1ng/tube×2 |
EK2215 -DA | Biotinylated anti- Human HEXA ELISA Kit A antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 528 | 1757 | 4955 | 552 | 1694 | 4751 |
Standard deviation | 20.59 | 89.61 | 237.84 | 28.70 | 76.23 | 242.3 |
CV(%) | 3.9 | 5.1 | 4.8 | 5.2 | 4.5 | 5.1 |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
O.D. | 0.025 | 0.068 | 0.145 | 0.297 | 0.461 | 0.955 | 1.432 | 2.077 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
蛋白名全称
Beta-hexosaminidase subunit alpha
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)