产品名称
Human Jagged1/JAG1 ELISA Kit
指标别名
JAG1; JAGL1; Protein jagged-1; Jagged1; hJ1; CD antigen CD339
免疫原
Expression system for standard: CHO; Immunogen sequence: S32-S1046
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4,000 pg/ml
基因名全称
jagged canonical Notch ligand 1
文献引用格式
Human Jagged1/JAG1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2224)
背景资料
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
试剂盒内容
Catalog number | Description | Quantity |
EK2224-CAP | 96-well plate precoated with anti- Human Jagged1 ELISA Kit A antibody | 1 |
EK2224-ST | Lyophilized recombinant Human Jagged1 ELISA Kit A standard | 10ng/tube×2 |
EK2224 -DA | Biotinylated anti- Human Jagged1 ELISA Kit A antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 90 | 185 | 1704 | 76 | 200 | 1687 |
Standard deviation | 2.79 | 12.91 | 48.39 | 4.92 | 10.12 | 69.34 |
CV(%) | 3.1% | 7% | 2.8% | 6.5% | 5.1% | 4.1% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
O.D. | 0.049 | 0.072 | 0.129 | 0.218 | 0.421 | 0.775 | 1.478 | 2.079 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA).