产品名称
Rat CD25/IL2RA ELISA Kit
指标别名
Il2ra Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; CD antigen CD25
免疫原
Expression system for standard: CHO; Immunogen sequence: E22-Q235
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
93.7 pg/ml - 6,000 pg/ml
基因名全称
interleukin 2 receptor subunit alpha
文献引用格式
Rat CD25/IL2RA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2271)
背景资料
CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
试剂盒内容
Catalog number | Description | Quantity |
EK2271-CAP | 96-well plate precoated with anti- Rat CD25/IL2RA ELISA Kit A antibody | 1 |
EK2271-ST | Lyophilized recombinant Rat CD25/IL2RA ELISA Kit A standard | 10ng/tube×2 |
EK2271 -DA | Biotinylated anti- Rat CD25/IL2RA ELISA Kit A antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 114 | 306 | 2233 | 155 | 316 | 2793 |
Standard deviation | 6.68 | 15.67 | 64.09 | 10.37 | 22.03 | 161.99 |
CV(%) | 5.9% | 5.1% | 2.9% | 6.7% | 7% | 5.8% |
典型数据示例
Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
O.D. | 0.019 | 0.084 | 0.156 | 0.395 | 0.581 | 0.881 | 1.275 | 1.831 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA).