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Rat CD25/IL2RA ELISA Kit

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EK2271

  • 96T ¥2480
  • 货期: 7-8周
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产品名称
Rat CD25/IL2RA ELISA Kit
指标别名
Il2ra Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; CD antigen CD25
免疫原
Expression system for standard: CHO; Immunogen sequence: E22-Q235
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
93.7 pg/ml - 6,000 pg/ml
基因名称
Il2ra
基因名全称
interleukin 2 receptor subunit alpha
Uniprot ID
P26897  
文献引用格式
Rat CD25/IL2RA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2271)
背景资料
CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK2271-CAP96-well plate precoated with anti- Rat CD25/IL2RA ELISA Kit A antibody1
EK2271-STLyophilized recombinant Rat CD25/IL2RA ELISA Kit A standard10ng/tube×2
EK2271 -DABiotinylated anti- Rat CD25/IL2RA ELISA Kit A antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)11430622331553162793
Standard deviation6.6815.6764.0910.3722.03161.99
CV(%)5.9%5.1%2.9%6.7%7%5.8%
典型数据示例
Concentration(pg/ml)093.7187.53757501,5003,0006,000
O.D.0.0190.0840.1560.3950.5810.8811.2751.831
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
rat
检测方法
酶联免疫双抗夹心法
Gene ID
25704  
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA).
实验时长
3.5h

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