产品名称
Canine CCL2/MCP-1 ELISA Kit
指标别名
C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2; CCL2; MCP1, SCYA2
免疫原
Expression system for standard: E.coli; Immunogen sequence: Gln24-Pro101
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 2
文献引用格式
Canine CCL2/MCP-1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2331)
背景资料
Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK2331-CAP | 96-well plate precoated with anti- Canine CCL2/MCP-1 antibody | 1 |
| EK2331-ST | Lyophilized recombinant Canine CCL2/MCP-1 standard | 10 ng/tube×2 |
| EK2331-DA | Biotinylated anti- Canine CCL2/MCP-1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 28 | 123 | 319 | 31 | 119 | 314 |
| Standard deviation | 2.00 | 9.27 | 13.40 | 1.90 | 4.94 | 11.74 |
| CV (%) | 7.2% | 7.5% | 4.2% | 6.1% | 4.2% | 3.7% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.006 | 0.097 | 0.209 | 0.452 | 0.791 | 1.278 | 1.763 | 2.161 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum, plasma(heparin, EDTA)
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