产品名称
Canine IL-10 ELISA Kit
指标别名
Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10
免疫原
Expression system for standard: E.coli; Immunogen sequence: Ser20-Ile179
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Receptor tyrosine-protein kinase erbB-2
文献引用格式
Canine IL-10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2335)
背景资料
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK2335-CAP | 96-well plate precoated with anti- Canine IL-10 antibody | 1 |
EK2335-ST | Lyophilized recombinant Canine IL-10 standard | 10 ng/tube×2 |
EK2335-DA | Biotinylated anti- Canine IL-10 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 52 | 136 | 514 | 62 | 155 | 721 |
Standard deviation | 3.84 | 7.75 | 23.28 | 4.74 | 7.41 | 15.21 |
CV (%) | 7.4% | 5.7% | 4.5% | 7.7% | 4.8% | 2.1% |
典型数据示例
Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.062 | 0.107 | 0.189 | 0.379 | 0.668 | 1.08 | 1.624 | 2.217 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum, plasma(heparin, EDTA)