产品名称
Canine IL-1 beta ELISA Kit
指标别名
Interleukin-1 beta; IL-1 beta; Il1b
免疫原
Expression system for standard: E.coli; Immunogen sequence: Ala114-Ser265
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Canine IL-1 beta ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2338)
背景资料
Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK2338-CAP | 96-well plate precoated with anti- Canine IL-1 beta antibody | 1 |
| EK2338-ST | Lyophilized recombinant Canine IL-1 beta standard | 10 ng/tube×2 |
| EK2338-DA | Biotinylated anti- Canine IL-1 beta antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 129 | 451 | 1884 | 162 | 494 | 1931 |
| Standard deviation | 6.90 | 27.01 | 72.16 | 5.38 | 38.88 | 56.58 |
| CV (%) | 5.4% | 6.0% | 3.8% | 3.3% | 7.9% | 2.9% |
典型数据示例
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.2 | 62.4 | 125 | 250 | 500 |
| O.D. | 0.05 | 0.157 | 0.259 | 0.481 | 0.836 | 0.1.339 | 1.923 | 2.255 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum, plasma(heparin, EDTA)
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