产品名称
Canine IL-12/IL-23 p40 ELISA Kit
指标别名
Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; Il12b
免疫原
Expression system for standard: NS0; Immunogen sequence: Ile23-Ser329
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Canine IL-12/IL-23 p40 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2340)
背景资料
Subunit beta of interleukin 12 (IL12B), also known as interleukin 12B, or interleukin-12 subunit p40, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK2340-CAP | 96-well plate precoated with anti- Canine IL-12/IL-23 p40 antibody | 1 |
EK2340-ST | Lyophilized recombinant Canine IL-12/IL-23 p40 standard | 10 ng/tube×2 |
EK2340-DA | Biotinylated anti- Canine IL-12/IL-23 p40 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 120 | 420 | 1250 | 67 | 439 | 1792 |
Standard deviation | 2.87 | 15.08 | 67.75 | 2.36 | 33.67 | 40.86 |
CV (%) | 2.4% | 3.6% | 5.4% | 3.5% | 7.7% | 2.3% |
典型数据示例
Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.06 | 0.095 | 0.134 | 0.198 | 0.322 | 0.598 | 1.084 | 1.858 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum, plasma(heparin, EDTA)