产品名称
Canine GM-CSF ELISA Kit
指标别名
Cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; CD131; Csf2rb; Aic2b; Csf2rb1; Il3rb1
免疫原
Expression system for standard: E.coli; Immunogen sequence: Ala18-Lys144
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Cone-rod homeobox protein
文献引用格式
Canine GM-CSF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2342)
背景资料
Cytokine receptor common subunit beta is a protein that in humans is encoded by the CSF2RB gene. It is mapped to 15 E1; 15 37.36 cM. The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).
试剂盒内容
Catalog number | Description | Quantity |
---|
EK2342-CAP | 96-well plate precoated with anti- Canine GM-CSF antibody | 1 |
EK2342-ST | Lyophilized recombinant Canine GM-CSF standard | 10 ng/tube×2 |
EK2342-DA | Biotinylated anti- Canine GM-CSF antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 203 | 1096 | 4354 | 310 | 1131 | 2853 |
Standard deviation | 14.74 | 82.75 | 94.92 | 7.50 | 89.35 | 132.95 |
CV (%) | 7.3% | 7.6% | 2.2% | 2.4% | 7.9% | 4.7% |
典型数据示例
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.005 | 0.083 | 0.165 | 0.336 | 0.688 | 1.24 | 2.074 | 2.369 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum, plasma(heparin, EDTA)