Canine CCL5/RANTES ELISA Kit

96T|2800

C-C motif chemokine 5; EoCP; Eosinophil chemotactic cytokine; SIS-delta; Small-inducible cytokine A5; T cell-specific protein P228; TCP228; T-cell-specific protein RANTES; RANTES (3-68); RANTES (4-68); CCL5; D17S136E, SCYA5

Expression system for standard: E.coli; Immunogen sequence: Ser24-Ser91

There is no detectable cross-reactivity with other relevant proteins.

<5 pg/mL

23.4-1,500 pg/mL

CCL5

C-C motif chemokine 5

Q8HYS0  

Canine CCL5/RANTES ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2344)

Chemokine (C-C motif) ligand 5 (also CCL5) is a protein which in humans is encoded by the CCL5 gene. It is also known as RANTES. This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

Catalog number	Description	Quantity
EK2344-CAP	96-well plate precoated with anti- Canine CCL5/RANTES antibody	1
EK2344-ST	Lyophilized recombinant Canine CCL5/RANTES standard	10 ng/tube×2
EK2344-DA	Biotinylated anti- Canine CCL5/RANTES antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	36	174	674	31	125	716
Standard deviation	1.97	7.85	34.31	1.93	9.13	14.68
CV (%)	5.5%	4.5%	5.1%	6.2%	7.3%	2.1%

Concentration (pg/ml)	0	23.4	46	93.8	187.5	375	750	1500
O.D.	0.03	0.14	0.242	0.424	0.77	1.165	1.678	2.15

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

canine

酶联免疫双抗夹心法

403522  

cell culture supernatants, serum, plasma(heparin, EDTA)

3.5h