Anti-Human Insulin Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Anti-Insulin monoclonal antibody, Polystyrene micro-well plate |
Human Insulin Standards (S1~S5) | 5 | - | Insulin (10,16,30,60,100mIU/L), 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300 |
HRP Conjugated anti-Human Insulin antibody | 1 | 6ml | HRP Conjugated anti-Human Insulin antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1%Proclin-300 |
Controls | 2 | - | 100% natural protein, 0.1%Proclin-300 |
20X Wash Buffer Concentrate | 1 | 15ml | 0.2M PBS containing 0.5% tween 20 |
Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5*-Tetramethylbenzidine |
Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
Plate Sealers | 2 | Piece | |