产品名称
Human JAM-A/F11R/Junctional Adhesion Molecule 1 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
指标别名
CD321; F11 receptor; F11R; JAM; JAM 1; JAM A; JAM1; JAMA; JAMA1; JCAM; Junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; PAM 1; Platelet adhesion molecule 1; Platelet F11 receptor
免疫原
Expression system for standard: E.coli; Immunogen sequence: S28-V238
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
46.9 pg/ml - 3,000 pg/ml
文献引用格式
Human JAM-A/F11R/Junctional Adhesion Molecule 1 EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ1349)
背景资料
Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
试剂盒内容
| Catalog number |
Description |
Quantity |
Storage of opened/reconstituted material |
| EZ1349-CA |
Mouse anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 monoclonal antibody (Capture Antibody) |
500 µl, 4 µg/mL
(recommended dilution 1:100) |
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
|
| EZ1349-RB-DA |
Biotinylated goat anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 polyclonal antibody (Detection Antibody) |
500 µl (recommended dilution 1:100) |
| AR1103 |
Avidin-Biotin-Peroxidase Complex (ABC) |
500 µl (recommended dilution 1:100) |
| EZ1349-RB-ST |
Lyophilized recombinant human JAM-A/F11R/Junctional Adhesion Molecule 1 standard |
10 ng/tube×3
|
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. |
典型数据示例
| Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.011 | 0.122 | 0.187 | 0.314 | 0.615 | 0.923 | 1.435 | 1.858 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 18 days in room temperature. Freeze upon receiving.)
需要自备的试剂
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。
样本类型
cell culture supernatants and serum.
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