Mouse IL-1 beta/IL1B Quick ELISA Kit

96T/3580

IL1 beta; Catabolin; IL 1; IL 1 beta; IL1 BETA; IL-1 beta; IL1- beta; IL1B; IL1beta; IL1F2; IL-1β; Interleukin 1 beta; interleukin 1; beta; Pro IL-1β; IL 1B; IL-1B

Expression system for standard: E.coli; Immunogen sequence: V118-S269

There is no detectable cross-reactivity with other relevant proteins.

<1 pg/ml

12.5 pg/ml - 800 pg/ml

IL1B

Interleukin-1 beta

P10749  

Mouse IL-1 beta/IL1B Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0394)

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

15-25分钟

Catalog number	Description	Quantity
FEK0394-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1
FEK0394-ST	Mouse IL-1 beta/IL1B standard	10 ng/tube×2
FEK0394-A	Mouse IL-1 beta/IL1B Antibody A	3ml
FEK0394-B	Mouse IL-1 beta/IL1B Antibody B	3ml
AR1106-1	Sample Diluent	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
PLA-SEA	Adhesive Plate Sealers	2

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	33	211	483	36	217	481
Standard deviation	1.74	10.33	34.29	2.19	14.75	35.59
CV (%)	5.3%	4.9%	7.1%	6.1%	6.8%	7.4%

Concentration (pg/ml)	0	12.5	25	50	100	200	400	800
O.D.	0.030	0.065	0.123	0.212	0.336	0.609	1.031	1.898

Store at 4℃ for 6 months.

mouse

酶联免疫双抗夹心法

16176  

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).