产品名称
Human ADAM15 Quick ELISA Kit
指标别名
ADAM 15; ADAM15; MDC 15; MDC15; Metargidin
免疫原
Expression system for standard: NS0; Immunogen sequence: D207-T696
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4,000 pg/ml
基因名全称
Disintegrin and metalloproteinase domain-containing protein 15
背景资料
Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
文献引用格式
Human ADAM15 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1484)
试剂盒内容
| Catalog number | Description | Quantity |
| FEK1484-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 |
| FEK1484-ST | Human ADAM15 standard | 10 ng/tube×2 |
| FEK1484-A | Human ADAM15 Antibody A | 3ml |
| FEK1484-B | Human ADAM15 Antibody B | 3ml |
| AR1106-1 | Sample Diluent | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 112 | 674 | 1454 | 110 | 704 | 1511 |
| Standard deviation | 5.04 | 53.24 | 59.61 | 7.04 | 64.06 | 64.97 |
| CV (%) | 4.5% | 7.9% | 4.1% | 6.4% | 9.1% | 4.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.061 | 0.083 | 0.109 | 0.185 | 0.404 | 0.664 | 1.373 | 2.079 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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