Anti-NRF2/NFE2L2 Antibody (Clone#OTI8A10)

Mouse monoclonal antibody

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筛选器： All WB

M00078-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of NRF2/NFE2L2 using anti-NRF2/NFE2L2 antibody (M00078-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat RH-35 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse Hepa1/6 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-NRF2/NFE2L2 antigen affinity purified monoclonal antibody (M00078-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NRF2/NFE2L2 at approximately 100-110 kDa. The expected band size for NRF2/NFE2L2 is at 68 kDa.
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产品名称

Anti-NRF2/NFE2L2 Antibody (Clone#OTI8A10)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; NRF2; NRF2; NFE2L2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

NFE2L2

克隆号

OTI8A10

宿主

Mouse

抗体亚型

IgG2a

免疫原

Human recombinant protein fragment corresponding to amino acids 330-605 of human NFE2L2 (NP_006155) produced in E.coli.

计算分子量

68 kDa

实际分子量

100-110 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

NFE2L2(nuclear factor (erythroid-derived 2)-like 2) also known as NRF2 or NFE2-RELATED TRANSCRIPTION FACTOR 2, is a transcription factor that in humans is encoded by the NFE2L2 gene. NFE2, NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. NFE2L2 induces the expression of various genes including those that encode for several antioxidant enzymes, and it may play a physiological role in the regulation of oxidative stress. The NFE2L2 gene is located on 2q31.2. The identification of somatic mutations that disrupt the NRF2-KEAP1 interaction to stabilize NRF2 and increase the constitutive transcription of NRF2 target genes indicated that enhanced reactive oxygen species (ROS) detoxification and additional NRF2 functions may in fact be tumorigenic. Oncogene-directed increased expression of Nrf2 is a mechanism for the activation of the Nrf2 antioxidant program evident in primary cells and tissues of mice expressing KRas(G12D) and BRaf(V619E), and in human pancreatic cancer.

Uniprot ID

Q16236

文献引用格式

NRF2/NFE2L2 Antibody (Clone#OTI8A10) (Boster Biological Technology, Wuhan, China. Catalog#M00078-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nuclear factor, erythroid 2 like 2

蛋白名全称

Nuclear factor erythroid 2-related factor 2

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