Anti-NRAS Antibody (Clone#OTI5G7)

Mouse monoclonal antibody

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筛选器： All WB IHC

M00099-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the 3 different overexpression plasmids (1:HRAS;2: NRAS; 3:KRAS) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-flag antibody (1:1000) or anti-NRAS mouse monoclonal antibody (Cat# M00099-3, 1:500).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRAS(Cat# M00099-3).
all(3)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00099-3)
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产品简介>

产品名称

Anti-NRAS Antibody (Clone#OTI5G7)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ALPS4; GTPase NRas; HRAS1; N ras; NRAS; NRAS1; Transforming protein N Ras

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

NRAS

克隆号

OTI5G7

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human NRAS (NP_002515) produced in HEK293T cell.

计算分子量

21 kDa

实际分子量

21 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

Uniprot ID

P01111

文献引用格式

NRAS Antibody (Clone#OTI5G7) (Boster Biological Technology, Wuhan, China. Catalog#M00099-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

GTPase NRas

蛋白名全称

GTPase Nras

实验方案>

实验步骤

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