Anti-HRAS Antibody (Clone#OTI1F2)

Mouse monoclonal antibody

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M00114-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HRAS monoclonal antibody at 1:200 (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
all(3)
Anti-HRAS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS .
all(3)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
all(3)

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产品简介>

产品名称

Anti-HRAS Antibody (Clone#OTI1F2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

C BAS/HAS; C H RAS; C HA RAS1; CTLO; GTPase HRas; H Ras 1; H RASIDX; Ha Ras; HAMSV; HRAS; HRAS1; HRAS-Specific; K RAS; RASH1; Transforming protein p21

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

HRAS

克隆号

OTI1F2

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human HRAS(NP_005334) produced in HEK293T cell.

计算分子量

21 kDa

实际分子量

21 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

GTPase HRas, also known as transforming protein p21, is an enzyme that in humans is encoded by the HRAS gene. This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

Uniprot ID

P01112

文献引用格式

HRAS Antibody (Clone#OTI1F2) (Boster Biological Technology, Wuhan, China. Catalog#M00114-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Harvey rat sarcoma viral oncogene homolog

蛋白名全称

GTPase Hras

实验方案>

实验步骤

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