Anti-Beclin 1/BECN1 Antibody (Clone#OTI3H8)

Mouse monoclonal antibody

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M00327-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00327-3)
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00327-3)
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BECN1 antibody, and then analyzed by flow cytometry.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00327-3)
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Anti-BECN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1 .
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
all(7)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Beclin 1/BECN1 Antibody (Clone#OTI3H8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ATG6; Beclin 1; beclin 1; autophagy related; beclin1; BECN1; GT197; Protein GT197; VPS30; Beclin-1

产品类型

Monoclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, IHC, ICC/IF, FCM

基因名称

BECN1

克隆号

OTI3H8

宿主

Mouse

抗体亚型

IgG2a

免疫原

Full length human recombinant protein of human BECN1 (NP_003757) produced in HEK293T cell.

计算分子量

52 kDa

实际分子量

51.7 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]). [supplied by OMIM, Jul 2010]

Uniprot ID

Q14457

文献引用格式

Beclin 1/BECN1 Antibody (Clone#OTI3H8) (Boster Biological Technology, Wuhan, China. Catalog#M00327-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

beclin 1, autophagy related

蛋白名全称

Beclin-1

实验方案>

实验步骤

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