Anti-4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) Antibody (Clone#32E52)

Rabbit monoclonal antibody

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筛选器： All WB IHC

M00968T46T32

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) antibody (M00968T46T32). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) antigen affinity purified monoclonal antibody (M00968T46T32) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) at approximately 18 kDa. The expected band size for 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) is at 18 kDa.
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产品名称

Anti-4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) Antibody (Clone#32E52)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I; EIF4EBP2; Eukaryotic translation initiation factor 4E-binding protein 2; 4E-BP2; eIF4E-binding protein 2; EIF4EBP3; Eukaryotic translation initiation factor 4E-binding protein 3; 4E-BP3; eIF4E-binding protein 3

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC

基因名称

EIF4EBP1/EIF4EBP2/EIF4EBP3

克隆号

32E52

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-eIF4EBP1/2/3 (T46+T46+T32)

计算分子量

18 kDa

实际分子量

18 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Uniprot ID

Q13541/Q13542/O60516

文献引用格式

4EBP1/EIF4EBP1/2/3 (Phospho-T46/T46/T32) Antibody (Clone#32E52) (Boster Biological Technology, Wuhan, China. Catalog#M00968T46T32)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

eukaryotic translation initiation factor 4E binding protein 1/2/3

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实验步骤

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