Western blot (WB): | 1:500-2000 |
Western blot analysis of anti-P-EIF4EBP1 antibody (M00968T70). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-EIF4EBP1 antigen affinity purified monoclonal antibody (M00968T70) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-EIF4EBP1 at approximately 17 kDa. The expected band size for P-EIF4EBP1 is at 17 kDa.
Western blot analysis of anti-P-EIF4EBP1 antibody (M00968T70). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-EIF4EBP1 antigen affinity purified monoclonal antibody (M00968T70) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-EIF4EBP1 at approximately 17 kDa. The expected band size for P-EIF4EBP1 is at 17 kDa.