Anti-Livin/BIRC7 Antibody (Clone#OTI1D12)

Mouse monoclonal antibody

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M02577

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Flow cytometric analysis of Hela cells, using anti-BIRC7 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BIRC7mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02577, Dilution 1:50)
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Flow cytometric analysis of Jurkat cells, using anti-BIRC7 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BIRC7 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-BIRC7 monoclonal antibody (1:200).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC7(Cat# M02577).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Livin/BIRC7 Antibody (Clone#OTI1D12)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

BIRC7; KIAP; LIVIN; ML IAP; MLIAP; RING finger protein 50; RNF50

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, FCM

基因名称

BIRC7

克隆号

OTI1D12

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human BIRC7 (NP_071444) produced in HEK293T cell.

计算分子量

32 kDa

实际分子量

33 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

0.36 mg/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide.

Uniprot ID

A2AWP0

文献引用格式

Livin/BIRC7 Antibody (Clone#OTI1D12) (Boster Biological Technology, Wuhan, China. Catalog#M02577)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

baculoviral IAP repeat-containing 7 (livin)

蛋白名全称

Baculoviral IAP repeat-containing protein 7

实验方案>

实验步骤

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