| Western blot (WB): | 1:500~2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FOLH1 monoclonal antibody.

Anti-FOLH1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOLH1 .

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-FOLH1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOLH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOLH1.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FOLH1 monoclonal antibody.

Anti-FOLH1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOLH1 .

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-FOLH1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOLH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOLH1.



