Western blot (WB): | 1:2000 |
Flow Cytometry (FCM): | 1:100 |
Flow cytometric analysis of TNFRSF18 expression on stimulated human peripheral blood mononuclear cells (PBMC). Unstimulated PBMC, (a.left, b.blue), or 10ug/ml Phytohemagglutinin-stimulated (3 days) PBMC, (a.right, b.red), were stained with Anti-Human TNFRSF18 antibody (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF18.
Flow cytometric analysis of TNFRSF18 expression on stimulated human peripheral blood mononuclear cells (PBMC). Unstimulated PBMC, (a.left, b.blue), or 10ug/ml Phytohemagglutinin-stimulated (3 days) PBMC, (a.right, b.red), were stained with Anti-Human TNFRSF18 antibody (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF18.