Anti-BAG2 Antibody (Clone#8F11G2)

筛选器： All WB FCM

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  Western blot analysis of BAG2 using anti-BAG2 antibody (M04933-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Jurkat whole cell lysates,
  Lane 2: Hela whole cell lysates,
  Lane 3: HepG2 whole cell lysates,
  Lane 4: K562 whole cell lysates,
  Lane 5: mouse liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BAG2 antigen affinity purified monoclonal antibody (M04933-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BAG2 at approximately 24 kDa. The expected band size for BAG2 is at 24 kDa.

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  Flow Cytometry analysis of K562 cells using anti-BAG2 antibody (M04933-2).
  Overlay histogram showing K562 cells stained with M04933-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-BAG2 Antibody (M04933-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of BAG2 using anti-BAG2 antibody (M04933-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Jurkat whole cell lysates,
  Lane 2: Hela whole cell lysates,
  Lane 3: HepG2 whole cell lysates,
  Lane 4: K562 whole cell lysates,
  Lane 5: mouse liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BAG2 antigen affinity purified monoclonal antibody (M04933-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BAG2 at approximately 24 kDa. The expected band size for BAG2 is at 24 kDa.

• 

  Flow Cytometry analysis of K562 cells using anti-BAG2 antibody (M04933-2).
  Overlay histogram showing K562 cells stained with M04933-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-BAG2 Antibody (M04933-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.